2021 IEEE International Conference on Image Processing

19-22 September 2021 • Anchorage, Alaska, USA

Imaging Without Borders

2021 IEEE International Conference on Image Processing

19-22 September 2021 • Anchorage, Alaska, USA

Login Paper Search My Schedule Paper Index Help

My ICIP 2021 Schedule

Note: Your custom schedule will not be saved unless you create a new account or login to an existing account.
  1. Create a login based on your email (takes less than one minute)
  2. Perform 'Paper Search'
  3. Select papers that you desire to save in your personalized schedule
  4. Click on 'My Schedule' to see the current list of selected papers
  5. Click on 'Printable Version' to create a separate window suitable for printing (the header and menu will appear, but will not actually print)

Paper Detail

Paper IDSS-CIMM.4
Paper Title SUPER-RESOLVED IMAGING OF EARLY-STAGE DYNAMICS IN THE IMMUNE RESPONSE
Authors Yair Ben Sahel, Gilli Dardikman-Yoffe, Yonina Eldar, Shirsendu Gosh, Gilad Haran, Weizmann Institute of Science, Israel
SessionSS-CIMM: Special Session: Computational Imaging for Materials and Microscopy
LocationArea B
Session Time:Monday, 20 September, 13:30 - 15:00
Presentation Time:Monday, 20 September, 13:30 - 15:00
Presentation Poster
Topic Special Sessions: Computational Imaging for Materials and Microscopy
IEEE Xplore Open Preview  Click here to view in IEEE Xplore
Abstract The use of photo-activated fluorescent molecules to create long sequences of low-density, diffraction-limited images gives us the ability to achieve highly-precise molecule localizations. However, this methodology requires lengthy imaging times, resulting in poor temporal resolution. This is particularly problematic when dynamic interactions of live cells on short time scales are of interest. We consider the problem of shortening dramatically the acquisition times in super-resolution microscopy down to seconds, in order to image the cellular dynamics during T-cell activation. For this purpose, we apply our newly developed method for super-resolution microscopy, SPARCOM, to real-time imaging of the immune response initiation. Despite the small number of frames used for reconstruction, we are able to achieve high-quality imaging that shows how T-cell receptors re-organize during the activation events, with respect to the cell's surface. Thus, our work demonstrates the use of SPARCOM for imaging dynamic cellular processes well below the diffraction limit.